RNA and OD260nm

Dr. Hiranya S. Roychowdhury hroychow at nmsu.edu
Thu Sep 19 14:57:58 EST 2002

Hmmm ... 

 If the absorbance reading of the sample at 260nm is "r", then you have 40r 
microgram of RNA per milliliter in the sample you are reading off. However, the 
accuracy of this simple relation is contingent upon the purity of the sample 
(A260:A280 = 2.0, ideally). 

Nothing surprises me aymore!

Quoting yoyo <yoyo.super at caramail.com>:

> Hi,
> Does anybody know how to calculate the quantite of RNA from the OD260nm
> value?
> Thanks in advance for your help
> Yoyo

Hiranya S. Roychowdhury, Ph.D.
Coll. Asst. Professor,
Molecular Biology,
Dept. of Chemistry & Biochemistry
Rm# 336, Chemistry Bldg.; MSC 3MLS
New Mexico State University
Las Cruces, NM 88003

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