MJ Thermal Cycler Tetrad problems

Dan Karney dmkarney at yahoo.com
Fri Sep 20 16:10:44 EST 2002


Our lab has 4 tetrads.  The bases themselves have performed fine,
however we are having problems with the heads.  The heads are not
performing correctly and our PCR reactions are producing no data. 
Sometimes we get error messages and sometimes not.  Has anyone else
had any problems?

We are also having problems with evaporation.  Any input would be
greatly appreciated.

We use ABI's 384 well-plates with ABI's (very expensive) optical
seals.

Thanks.

Dan Karney
Comparative Genomics Lab
Arizona Respiratory Center
Tucson, AZ



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