IEF and glycoproteins staining

Dr Engelbert Buxbaum engelbert_buxbaum at
Fri Apr 11 02:16:51 EST 2003

dabram wrote:

> Hello all! I have a little problem,  I don't know how to stain
> glycoproteins into the isoelectric focusing gel (IEF). Is anyone who
> did it?

Have you tried PAS (periodic acid Schiff)-staining already? For SDS-PAGE
a method is described in Meth. Enzymol. 28 (1972) 54-63, after thorough
fixing and washing it should also work with IEF gels. PAS is specific
for glycoprotein, if you want all proteins, silver or Coomassie
Brilliant blue are also possible.

More information about the Methods mailing list