IEF and glycoproteins staining

Dr Engelbert Buxbaum engelbert_buxbaum at hotmail.com
Fri Apr 11 02:16:51 EST 2003


dabram wrote:

> Hello all! I have a little problem,  I don't know how to stain
> glycoproteins into the isoelectric focusing gel (IEF). Is anyone who
> did it?

Have you tried PAS (periodic acid Schiff)-staining already? For SDS-PAGE
a method is described in Meth. Enzymol. 28 (1972) 54-63, after thorough
fixing and washing it should also work with IEF gels. PAS is specific
for glycoprotein, if you want all proteins, silver or Coomassie
Brilliant blue are also possible.



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