PCR trouble with Pfu

[Luk Vandenberghe]

Duncan Clark wrote:
> Historians believe that in newspost <3E96E3C6.1020304 at REMOVEua.es> on 
> Fri, 11 Apr 2003, Rafael Maldonado <rmaldonado at REMOVEua.es> penned the 
> following literary masterpiece:
> 
>> Expand, one of the enzymes used, is a mixture of Taq and something 
>> they don't tell you.
> 
> 
> Pwo.


In the US they had to replace Pwo with Tgo for patent reasons. I was 
told that Pwo and Pfu were actually identical as to their AA sequence; 
not sure if that is right. In my hands the Pwo Expand mix has always 
worked great; the Tgo unfortunatly does not live up to that anymore. I 
had very mixed results with Pfu as well in situations were the Taq 
worked fine.

Anyways if somebody knows how to get a hold of Pwo in the US, please let 
me know.

L