generating standard curve for quantitate human whole blood RNA

zhuhua9863 at yahoo.com zhuhua9863 at yahoo.com
Wed Apr 30 14:25:08 EST 2003


Does anyone can give me idea about selecting right template to generate standard curve for quantify two genes of human whole blood by multiplexing real-time PCR? If you have no specifically designed primers(by one base mutation, e.g) to amplify same gene from two different templates(one is your unknown sample, another one is for internal control), then you have to amplify two genes from same template by using two different pairs of primers. What is the best way to generate a standard curve for normalization?

Thank you for help!   


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