Nuclei friendly buffer.

Wolfgang Schechinger hubahopp at gmx.de
Sun Aug 24 05:26:21 EST 2003


Dear Julia, 

check here:
http://www.google.de/search?hl=de&ie=UTF-8&oe=UTF-8&q=nfkb+staining&btnG=Goo
gle+Suche&meta=
and there: http://www.ncbi.nih.gov/entrez/query.fcgi 

Regards, 

Wo


At 00:29 24.08.2003 -0400, Julia wrote:
>Hi, everyone,
>
>I am trying to stain NFkB in the nuclei, and my protocol does not specify
>what buffer I should use for staining itself.
>I was using PIPES buffer to extract the nuclei, then I used PBS to dissolve
>my antibodies.
>I think that I lost most of the nuclei at this step.
>Does anyone has a recipe for a nuclei friendly buffer (preferably for
>staining).
>
>Thank you in advance,
>
>Julia
>
>
>
>

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