Nuclei friendly buffer.
hubahopp at gmx.de
Sun Aug 24 05:26:21 EST 2003
and there: http://www.ncbi.nih.gov/entrez/query.fcgi
At 00:29 24.08.2003 -0400, Julia wrote:
>I am trying to stain NFkB in the nuclei, and my protocol does not specify
>what buffer I should use for staining itself.
>I was using PIPES buffer to extract the nuclei, then I used PBS to dissolve
>I think that I lost most of the nuclei at this step.
>Does anyone has a recipe for a nuclei friendly buffer (preferably for
>Thank you in advance,
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