Nuclei friendly buffer.

Wolfgang Schechinger hubahopp at
Sun Aug 24 05:26:21 EST 2003

Dear Julia, 

check here:
and there: 



At 00:29 24.08.2003 -0400, Julia wrote:
>Hi, everyone,
>I am trying to stain NFkB in the nuclei, and my protocol does not specify
>what buffer I should use for staining itself.
>I was using PIPES buffer to extract the nuclei, then I used PBS to dissolve
>my antibodies.
>I think that I lost most of the nuclei at this step.
>Does anyone has a recipe for a nuclei friendly buffer (preferably for
>Thank you in advance,


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