SDS-Agarose electrophoresis of HMW proteins

[tvink]

Hi,

Trie looking for electrophoresis of large proteins. I myself did use
it for von Willebrand factor (up to 10 million dalton). I also used
blotting on these gels and that worked fine. There must be lots of
literature on this subject just do a pubmed search I suppose.

Grtz Tom


On 4 Dec 2003 10:20:42 -0800, microlaserpro at lycos.com (David Micklem)
wrote:

>Hi,
>
>Someone here mentioned recently the use of SDS-Agarose gel
>electrophoresis for separation of large proteins (I am interested in
>>>500kDa proteins/complexes).
>
>Can anyone point me to a good practical protocol for this method? Its
>not in any of the general lab manuals that I've been able to lay my
>hands on. Any comments on general limitations of the method compared
>to SDS-PAGE?
>
>I am particularly interested to know if it is possible to run
>'diagonal' 2D gels (non-reducing 1st dimension, reducing 2nd
>dimension) in SDS-agarose. Can the gels be stained/blotted/etc the
>same way that SDS-PAGE gels are? What is the approximate range of
>sizes that can be separated on a single gel - presumably that is
>dependent on the agarose concentration?
>
>AdvTHANKSance,
>
>David