p53 responsive vectors
ucgatan at socrates-a.ucl.ac.uk
Tue Dec 16 15:03:59 EST 2003
On Mon, 15 Dec 2003, Ice9 wrote:
> "Tom Anderson" <ucgatan at socrates-a.ucl.ac.uk> wrote in message
> news:Pine.SOL.4.33.0312151255300.6388-100000 at socrates-a.ucl.ac.uk...
> > On Mon, 15 Dec 2003, Ice9 wrote:
> > > In summary, what I'd like to do, is to have a p53 responsive
> > > expression vector capable of expressing my protein.
> > Just out of curiosity, why p53 in particular?
> For several reasons... (a) the gene of interest is p53 responsive
This is almost certainly a stupid question, but if the gene is already
p53-responsive, why do you need to make a construct with it under a p53
response element? And if you do need it as a separate construct, how about
using its own promoter?
If, not, is the TRANSFAC database any use? You can search it for
transcription factor binding sites:
It suggests a few p53 response elements, and the record for each points to
the paper describing it.
Tom Anderson, MRC Laboratory for Molecular Cell Biology, UCL
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