can ligase cut DNA?
nobody at elnino.com
Mon Dec 22 15:18:33 EST 2003
"Nick Theodorakis" <nick_theodorakis at hotmail.com> wrote in message
news:f4f2b038.0312220948.35414796 at posting.google.com...
> dikla_dar at walla.co.il (diki) wrote in message
news:<6d45091f.0312210501.23a4cb02 at posting.google.com>...
> > hi everybody!
> > my question is maybe silly, but i need to know: can ligase cut
> > (instead of ligate) any kind of DNA or RNA (ds or ss) under any
> > circumstances?
> > thank u.
> The reading is from Kornberg, chapter 8, section 3 (p. 268):
> "As already indicated, each of the covalent intermediates participates
> in a reverse reaction. Ligase-adenylate generates NAD (or ATP) from
> NMN (or PPi); DNA-adenylate reacts with free ligase and NMN to form
> NAD. Reversal of the entire sequence of reactions can be demonstrated
> in the AMP-dependent conversion of a covalently closed, superhelical
> circle via a nicked, adenylated form to a relaxed circle which may be
> coalently closed (Fig. 8-4)."
> Here endeth the reading.
>  Kornberg, A. _DNA_Replication_ (1980)
Which simply translates, I guess, use highest purity reagents in your
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