can ligase cut DNA?

Duncan Clark junk at hgmp.mrc.ac.uk
Tue Dec 23 04:51:27 EST 2003


Historians believe that in newspost 
<vkIFb.19$_4.11171 at news.uchicago.edu> on Mon, 22 Dec 2003, EK 
<nobody at elnino.com> penned the following literary masterpiece:
>Which simply translates, I guess, use highest purity reagents in your
>experiments. :-)

Won't necessarily help, because 'any' reaction can be forced into 
reverse i.e. pyrophosphorolysis in DNA sequencing by a DNA polymerase.

But as to ligase cutting DNA, one needs to define what is meant by 
cutting.

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.



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