Restriction Digest of MRSA plasmid

[Duncan Clark]

Historians believe that in newspost <b3331e$rgh$1 at wotsit.aston.ac.uk> on 
Thu, 20 Feb 2003, Anthony C. Hilton <a.c.hilton at aston.ac.uk> penned the 
following literary masterpiece:
>As a positive control we have been
>digesting in parallel a pBR322 derivative which cuts up nicely.  Any
>suggestions as to why our MRSA plasmid is not cutting, and how we might over
>come this?

Mix pBR322 with your MRSA plasmid and re-digest. If pBR322 cuts then 
your MRSA plasmid has no sites for that enzyme.

If the pBR322 doesn't cut then you have an inhibitor in your MRSA 
plasmid batch and you will need to clean that up.

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.