It is so strange

Carnac carnac at
Mon Jan 13 01:45:28 EST 2003

"Mike" <zhuhu at> wrote in message
news:avtic5$1ls8$1 at
> Hi,
> I met a strange problem, Could someone help me?
> I used the Plasmid Prep Kit of Qiagen to prepare the plasmids, but I found
> that there always have two small bands before the supercoiled plasmid
> The large one is about 500bp. Other plasmids also have this problem.  I do
> it strictly according to the manual.

Most likely contaminating OC plasmids.

More information about the Methods mailing list