Problem with Isoelectric Focusing on gels

Dr Engelbert Buxbaum engelbert_buxbaum at
Mon Jul 7 10:07:19 EST 2003

Christian Collin-Hansen wrote:

> Question: Is it nessecary to fix IEF-gels in TCA after focusing (prior
> to staining)? Are there alternative ways of fixing the gel?

4% Formaldehyde or (possibly better, but more expensive) 1%
glutardialdehyde can crosslink proteins and make them insoluble.

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