immunoprecipitation and IEF

Stephan Bartsch sbartsch at ucdavis.edu
Mon Jul 21 21:11:28 EST 2003


Folks,
I immunoprecipitate my protein of interest using Protein A.G Sepharose 
beads. Since I want to further analyse the protein of interest using 1D 
IEF I am looking for the best method to release the protein of interest 
from the IP-antibody protein A/G sepharose complex for subsequent 
analysis. SDS can not be used because of the negative charge interfering 
with IEF. Appreciate advice!

Regards

Stephan Bartsch, Ph. D.
Section of Microbiology 
Life Sciences Addition, Room 3143
University of California, Davis
Davis, CA 95616-8665

Phone Lab: (530) 752-3016
e-Mail: sbartsch at ucdavis.edu
                                  
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