Will lyophilisation kill all bacteria?

Wolfgang Schechinger hubahopp at gmx.de
Wed Jul 23 13:19:55 EST 2003


Will flushing the filter with BSA, gelatin, skim milk or whatever minimize
the adhesion of target protein to the membrane?

Just a thought

Wo


At 18:40 23.07.2003 +0100, Jayakumar wrote:
>Filter sterilization is also a good idea. but take care to use filters made
>of materials which will not cause the proteins to adhere to the filter.
>jayakumar
>
>> From: Peter Frank <peter_frankde at yahoo.de>
>> Reply-To: peter_frankde at yahoo.de
>> Date: Wed, 23 Jul 2003 18:49:21 +0200
>> To: methods at hgmp.mrc.ac.uk
>> Subject: Will lyophilisation kill all bacteria?
>> 
>> I made a protein solution which was supposed to be sterile because I
>> use it in cell culture experiments. However, it turned out it somehow
>> got contaminated by bacteria. The solution cannot be autoclaved (this
>> will irreversibly denature my protein), so I am looking for another
>> way to get my solution sterile. So my question is:
>> Will lyophilisation kill all bacteria that got into my solution and
>> anywhere in the tube?
>> 
>> Regards,
>> Peter
>
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