Conservative changes - glycosylated N
Ian A. York
iayork at panix.com
Thu Jul 24 09:42:12 EST 2003
In article <abmvhv07iof5qjq7kkvikh4akv7u74k8fv at 4ax.com>,
tvink <lullo at xs4all.nl> wrote:
>On Wed, 23 Jul 2003 16:00:33 +0000 (UTC), iayork at panix.com (Ian A.
>>I want to mutate away potential glycosylation sites while doing as little
>>as possible to the protein in general. I've seen lists of conservative
>>amino acid changes, but they don't differentiate between glycosylated
>>and non-glycosylated Ns. It seems to me that the most conservative
>>change may not be the same for the glycosylated and non-glycosylated Ns.
>>Can anyone point me to a reference that explains my options?
>If your are talking about N-linked glycosylation sites. Consensus
>NXS/T. The most logical would be a N to Q substitution I suppose.
Why? Do you have a reference for that?
Ian York (iayork at panix.com) <http://www.panix.com/~iayork/>
"-but as he was a York, I am rather inclined to suppose him a
very respectable Man." -Jane Austen, The History of England
More information about the Methods