Conservative changes - glycosylated N

Ian A. York iayork at panix.com
Thu Jul 24 09:42:12 EST 2003


In article <abmvhv07iof5qjq7kkvikh4akv7u74k8fv at 4ax.com>,
tvink  <lullo at xs4all.nl> wrote:
>On Wed, 23 Jul 2003 16:00:33 +0000 (UTC), iayork at panix.com (Ian A.
>York) wrote:
>>I want to mutate away potential glycosylation sites while doing as little 
>>as possible to the protein in general.  I've seen lists of conservative 
>>amino acid changes, but they don't differentiate between glycosylated 
>>and non-glycosylated Ns.  It seems to me that the most conservative 
>>change may not be the same for the glycosylated and non-glycosylated Ns.  
>>Can anyone point me to a reference that explains my options?
>
>If your are talking about N-linked glycosylation sites. Consensus
>NXS/T. The most logical would be a N to Q substitution I suppose.

Why?  Do you have a reference for that?

Ian
-- 
    Ian York   (iayork at panix.com)  <http://www.panix.com/~iayork/>
    "-but as he was a York, I am rather inclined to suppose him a
     very respectable Man." -Jane Austen, The History of England



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