cDNA from ds RNA

dr_pandu at yahoo.co.in dr_pandu at yahoo.co.in
Sat Jul 26 07:26:12 EST 2003


Dear netters,
 I am trying to synthesize cDNA from the genome of Blue tongue virus
-a Reo virus with double straded RNA. In most of the protocols the
authors used Methyl mercuric Hydrxide for denaturation of ds RNA
before going for cDNA stynthesis using RT. I could not get Methyl
mercuric Hydroxide from my chemical suplier. I would like to know
whether denaturation of RNA is essential for cDNA synthesis from
double stranded RNAs? Please sugest an alternative.
with regards
pandu



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