No ligation of PCR product in pGemT-Easy
junk at hgmp.mrc.ac.uk
Tue Jul 29 03:18:30 EST 2003
Historians believe that in newspost
<1059433320.3f25ab68ef94e at webmail.nmsu.edu> on Tue, 29 Jul 2003, Dr.
Hiranya S. Roychowdhury <hroychow at nmsu.edu> penned the following
>There is one basic thing you may have overlooked! Did you phophorylate the
>primers (prior to PCR) or the amplified products before ligation?
Shouldn't need to for TA cloning - Vector already has a terminal 5'
I love deadlines. I especially like the whooshing noise they make as
they go flying by.
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