No ligation of PCR product in pGemT-Easy

Duncan Clark junk at hgmp.mrc.ac.uk
Tue Jul 29 03:18:30 EST 2003


Historians believe that in newspost 
<1059433320.3f25ab68ef94e at webmail.nmsu.edu> on Tue, 29 Jul 2003, Dr. 
Hiranya S. Roychowdhury <hroychow at nmsu.edu> penned the following 
literary masterpiece:
>There is one basic thing you may have overlooked!  Did you phophorylate the
>primers (prior to PCR) or the amplified products before ligation?

Shouldn't need to for TA cloning - Vector already has a terminal 5' 
phosphate.

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.



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