No ligation of PCR product in pGemT-Easy

Duncan Clark junk at hgmp.mrc.ac.uk
Thu Jul 31 14:36:09 EST 2003


Historians believe that in newspost <3f294675$1 at news.unimelb.edu.au> on 
Thu, 31 Jul 2003, Vladimir Grubor <vladimirgrubor@?.?.com.invalid> 
penned the following literary masterpiece:
>Not sure about the Taq you are using, is it a mix with a proofreader?
>Because enzymes which have proofreading activity don't leave overhands which
>the pGEM kit relies on.

Very dependant upon which mix is being used. If the ration of Taq: 
Proofreader is high (10-20:1) we find the overhang is left on fine or 
shall we say we still get our TA clones without problem.

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.



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