Cell Cycle Markers (OT?)

Ian A. York iayork at panix.com
Wed Jun 18 10:03:49 EST 2003


In article <3EF07CD4.3010100 at yahoo.com>,
MA Bonetti  <mabonetti at yahoo.com> wrote:
>Hi everybody,
>
>I hope my question isn´t too much off-topic, but I didn´t found any 
>(popular) group I could have posted it....
>Actually I´ve made some GFP-fused constructs and I have the feeling, 
>that localization is somehow cell-cycle-dependent.
>How can I determine, without having to buy Antibodies for several 
>hundred dollars, if my cells are in G1, S etc...? Are there any suitable 
>"sampler kits", which would feature those needed AB?
>Is there maybe another way?

Flow cytometry of cells stained with propidium iodide can determine cell 
cycle status by quantifying the DNA content.  It's a standard protocol; 
check with your friendly local flow cytometry facilty.

Ian 
-- 
    Ian York   (iayork at panix.com)  <http://www.panix.com/~iayork/>
    "-but as he was a York, I am rather inclined to suppose him a
     very respectable Man." -Jane Austen, The History of England



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