Cell Cycle Markers (OT?)
Ian A. York
iayork at panix.com
Wed Jun 18 10:03:49 EST 2003
In article <3EF07CD4.3010100 at yahoo.com>,
MA Bonetti <mabonetti at yahoo.com> wrote:
>I hope my question isn´t too much off-topic, but I didn´t found any
>(popular) group I could have posted it....
>Actually I´ve made some GFP-fused constructs and I have the feeling,
>that localization is somehow cell-cycle-dependent.
>How can I determine, without having to buy Antibodies for several
>hundred dollars, if my cells are in G1, S etc...? Are there any suitable
>"sampler kits", which would feature those needed AB?
>Is there maybe another way?
Flow cytometry of cells stained with propidium iodide can determine cell
cycle status by quantifying the DNA content. It's a standard protocol;
check with your friendly local flow cytometry facilty.
Ian York (iayork at panix.com) <http://www.panix.com/~iayork/>
"-but as he was a York, I am rather inclined to suppose him a
very respectable Man." -Jane Austen, The History of England
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