Taq DNA Polymerase and 5'-3' Exonuclease activity

Wolfgang Schechinger hubahopp at gmx.de
Sat Mar 1 05:01:08 EST 2003

You mean, 

what happens if one incubates linear dsDNA with Taq at 72 degC?

Anyone tried?


At 03:10 PM 2003/2/28 -0800, Tired Khan wrote:
>Does anyone know whether the 5' to 3' exonuclease activity of DNA
>polymerases (in particular Taq) depends totally upon extension of a
>primer or can the activity exist in the absence of polymerization? Any
>studies at all on this? Any info on polymerases other than Taq would
>also be useful
>Many thanks


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