Health problem with methanol?

Dr Engelbert Buxbaum engelbert_buxbaum at hotmail.com
Wed Mar 5 03:59:41 EST 2003


Sean Patterson wrote:

> >For better blotting results, use Dunn's buffer system (10 mM NaHCO3, 3
> >mM Na2CO3, with MeOH for small protein and 50 uM SDS for big ones)
> >rather than the usual Towbin. Cheaper too :-)
> 
> Is that just for semi-dry, or does it work with wet blotting as well?


Works like a charm for tank blotters too (indeed, with the large
membrane proteins I deal with, that's the better option anyway). About
40-50 V const voltage for 2 h, cooled with an ice pack.



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