transferrin

Lisa Corcoran Lisa_Corcoran at onaustralia.com.au
Fri Mar 7 22:03:45 EST 2003


To all molecular biologists out there:
I am transfecting COS1 cells with GFP labelled transferrin receptor. I
normally transfect 10 ug of DNA. Is that appropriate for transferrin
receptor?
I also need to chase using holo transferrin but need to know how much
transferrin to add. The COS1 cells will also be transiently transfected with
10 ug of our protein of interest in a pEFBOS vector with flag tag and I will
come in with an antiboddy conjugated to a red choromo.
Any advice will be greatfully received.

Lisa





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