DNA polymerases free of bacterial (DNA) contamination

Wolfgang Schechinger hubahopp at gmx.de
Wed Mar 12 10:58:36 EST 2003


Just a thought:

contaminating DNA should be double stranded

Pfu has exonuclease activity

will the presence of some Pfu digest the contaminating DNA?

Wo


At 07:20 AM 2003/3/12 -0800, Mashounya wrote:
>Thanks to all!
>But the problem is still actual. I've tried to treat dirty Taq in a
>mix with buffer and dH2O with UV - it's effective in case of low
>contamination, but Taq's activity decreases dramatically.
>Any more ideas?
>
>

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