Why single colony?

EdiSon chang_edison at hotmail.com
Thu Mar 13 06:40:54 EST 2003


After identifying the positive clones from the cell transformation
plates, why do we still need to streak and pick single colony for DNA
miniprep and protein? Don't all the decedents from that single
positive clone  have the same genetic made-up? What's the things we
try to avoid? Mutation in the cloned gene? Hetergeneous mixture of
plamids? What? Please be kind and blow up my super small brain. Thank
you for now! Cheers, EdiSon.



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