Protocol for identification of unknown bacteria

Trond Erik Vee Aune trondaun at
Fri Mar 14 10:06:44 EST 2003


I want to know how to do 16s sequencing on an unknown gram negative 
bacteria. Preferably I would like to do the sequencing reaction directly 
on colonies without the need to isolate genomic DNA first. I would also 
like to know the sequence of the "universal" primers used. Is it enough 
with only one set of primers to get enough sequence to correctly 
identify the bacteria?

Trond Erik Vee Aune

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