Protocol for identification of unknown bacteria

Scott J. Coutts scott.coutts at med.monash.edu.au
Fri Mar 14 20:12:20 EST 2003


I asked someone at the Baker about it...

Scott J. Coutts wrote:
> Yeah, I just got that too. Hey Des, you're not at the Baker Institute at 
> the moment are you? I know your email address isnt a baker mail adress, 
> but I'm from Monash and I recieved that same email as you but it says 
> it's from "Khoa.Ngo at med.monash.edu.au" (which is the same domain as 
> where I mail from).
> 
> Wierd. I dont know how the comes back from a newsgroup post?! It didnt 
> even go by email.
> 
> Des O'Connor wrote:
> 
>> HI Again
>>
>> What is this about it came to me after I posted to you
>>
>> From: "Khoa Ngo" <Khoa.Ngo at baker.edu.au>
>> To: <des at genelab.plus.com>
>> Sent: Friday, March 14, 2003 6:00 PM
>> Subject: Re: Protocol for identification of unknown bacteria (Newcontact
>> details)
>>
>>
>> Khoa Ngo is no longer a Baker employee.
>>
>> If your Email concerns *** BLANK NAME OF INSTITUTE***  business please
>> resend it to the most appropriate Institute representative or, if you 
>> do not
>> know the Email address of the most appropriate person, resend it to
>>
>>
>> "Des O'Connor" <des at genelab.plus.com> wrote in message
>> news:wLoca.302$6f1.17679 at stones.force9.net...
>>
>>
>>
>>> HI Trond
>>>
>>> Im no expert in this area of molecular biology  but isnt  16S sequencing
>>> done on ribosommal RNA usually after density gradient centrifugation 
>>> thus
>>> automatically dispensing withe presence of the microbial  chromosome.
>>>
>>> I dont want to be a ''party pooper'' but Im pretty certain if your
>>
>>
>> starting
>>
>>> from scratch and dont have the correct tech ( ribotyping sequencer etc)
>>
>>
>> then
>>
>>> I suspect you have some months of hard work ahead and a large bill at 
>>> the
>>> end of it.
>>>
>>>
>>> On a more positive note if you wish to email me privately Ill run the 
>>> bug
>>> through our diagnostic lab (gratis) for you if that would help.
>>>
>>> Best Des
>>>
>>>
>>> "Trond Erik Vee Aune" 
>>> <trondaun at biotech.REMOVETHISBEFOREREPLYING.ntnu.no>
>>> wrote in message
>>> news:3E71F004.8000008 at biotech.REMOVETHISBEFOREREPLYING.ntnu.no...
>>>
>>>> Hi
>>>>
>>>> I want to know how to do 16s sequencing on an unknown gram negative
>>>> bacteria. Preferably I would like to do the sequencing reaction 
>>>> directly
>>>> on colonies without the need to isolate genomic DNA first. I would also
>>>> like to know the sequence of the "universal" primers used. Is it enough
>>>> with only one set of primers to get enough sequence to correctly
>>>> identify the bacteria?
>>>>
>>>> Trond Erik Vee Aune
>>>>
>>>
>>>
>>
>>
> 
> 


-- 
Scott J. Coutts
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