Protocol for identification of unknown bacteria

Artem Evdokimov AEVDOKIMOZ at cinci.rr.com
Sat Mar 15 22:03:48 EST 2003


Bacterial 16S forward primer: 5'-CCGAATTCGTCGACAACAGAGTTTGATCCTGGCTCAG-3'

Bacterial 16S reverse primer: 5'-CCCGGGATCCAAGCTTACGGCTACCTTGTTACGACTT-3'

for ~1500 bp of DNA.

Use at your own risk, it's always best to read the original article.

Artem

"Trond Erik Vee Aune" <trondaun at biotech.NOSPAM.ntnu.no> wrote in message
news:3E731890.1080008 at biotech.NOSPAM.ntnu.no...
>
>
> Subhash Kulkarni wrote:
> > the sequence of universal primers would depend upon how much u wanna
> > sequence....500, 1000 or 1500 bp......typical universal primers should
> > be available from ur company for the amount of bases u wanna sequence
>
> Thanks for your input. Would 500 bp be enough for a 100% accurate
> identification, or enough to submit to a 16s database if the bacterium
> is new to science?
>
> Trond Erik
>
> >
> > Trond Erik Vee Aune wrote:
> >
> >>
> >> Thanks for yet another very helpful answer Scott :)
> >>
> >> This bug is the same as the one from the "wierd bug"-thread at
> >> sci.bio.microbiology and bionet.microbiology. It's just something I'm
> >> working on beside of my regular project. I try not to spend too much
> >> time on it since I'm quite busy with other more important things, but
> >> it is very fun to try to characterize this wierdo. Since it is
> >> producing some kind of antibiotic it is even more interesting. But
> >> since I'm so busy with other things the progression is rather slow ;)
> >>
> >> Do you have the exact sequence of the "universal primers"? Cause I
> >> would prefer do order it from my regular company.
> >>
> >> Trond Erik
> >>
> >>
> >
> >
>





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