Alternative to RNAi? Liposome not needed for tranfection

Walter Schick - BioSepCo walterschick at ca.astound.net
Wed Mar 19 18:04:02 EST 2003


There are at least a dozen papers using electroporation for siRNA
transfection, and a leading supplier of has designed systems for in-vivo
delivery in animals and clinical studies.  The Cyto Pulse combination of a
high voltage pulse for poration and then low voltage electrophoresis-like
pulses for delivery has very low toxicity and thus gives high cell viability
and efficiency.

Check out http://www.cytopulse.com/invivo.html for gene delivery (or siRNA)
and also their systems for general and animal research.  They also have cell
fusion systems that work.

Walt Schick
BioSepCo
----- Original Message -----
From: "EK" <khatipovNO-SPAM at NO-SPAMuchicago.edu>
To: <methods at hgmp.mrc.ac.uk>
Sent: Wednesday, March 19, 2003 12:51 PM
Subject: Alternative to RNAi?


> I just wonder why everybody rushed these days into RNAi business, when a
> definite disadvantage of the technology is that it is inapplicable for in
> vivo studies because it requires liposome or otherwise mediated
> transfection.
> Would not that be easier just to make RNA fusions to protein transduction
> peptides like TAT? Do you guys know if it is possible to chemically link
an
> RNA (or DNA) chain to a linear peptide? Such RNA/peptide conjugates could
be
> directly administered to patients to silence gens...
> -Emir
>
>


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