Southern Blot

Sergio yokese at yahoo.com
Mon Mar 24 04:54:59 EST 2003


Are you using DIG?.
If doing genomic southerns, are you performing the depurinization step?.
Maybe you'd want to check the labelling and the specificity of your 
probe by some dot-blot analysis before trying the southern.

Sergio

Ong wrote:
> when hybridize my sample with non radioactive labeled peobe, only the DNA
> marker give signal. Why???? any one can help me???
> 
> 




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