RNA purification : polysacharides problems

benali at ensam.inra.fr benali at ensam.inra.fr
Mon Mar 31 10:25:52 EST 2003

I'm using the Fast RNA GREEN kitand the Fast Prep 120 Apparatus (Qbiogene) to purify RNA from wheat seeds. My samples are wheat seeds in different maturity stages (from few days after anthesis 'till total maturity). The mature seeds contain large amount of polysaccharids which make difficult to resuspend the RNA pellet. Does anybody have a suggestion about this? 


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