RNA purification : polysacharides problems
Subhash.Kulkarni at nospam.ie-zea.unil.ch
Mon Mar 31 10:35:38 EST 2003
this happens sometimes with my DNA, i do it by running the solution a
bit on the gel and then isolating it back by gel purification, i guess u
can work with a DEPC treated Gel and Buffer and do the same..........
benali at ensam.inra.fr wrote:
> I'm using the Fast RNA GREEN kitand the Fast Prep 120 Apparatus (Qbiogene) to purify RNA from wheat seeds. My samples are wheat seeds in different maturity stages (from few days after anthesis 'till total maturity). The mature seeds contain large amount of polysaccharids which make difficult to resuspend the RNA pellet. Does anybody have a suggestion about this?
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