Phosphoserine/threonine immunoblotting

Wolfgang Schechinger hubahopp at gmx.de
Mon Nov 3 04:56:24 EST 2003


Tom, 

to your question of obtaining good anti pS and pT:

Try to get hold of as many different abs as you can and test them. If you
can't find enough candidates on your floor or in your institute, just ask
the companies for (free) test aliquots. 

On the other hand, if you have the technical opportunity to run a 32P
metabolic labelling, just do it. 32P is (IMHO) much less dangerous than 3H
or C14, since you always can detect it and it's easy to shield off the
radiation.

Maybe it's helpful: I am vaguely aware of some papers by Strack-V (among
others) on the phosphorylation of insulinreceptor substrate. You might
check the methods sections.

Best, 

Wo



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