Plasmid extraction kits
R.Jayakumar at RoswellPark.org
Wed Nov 26 13:38:01 EST 2003
When I used to use quiagen mini prep kits during my PhD program back in India, our lab members often used to use the columns from the older kits in the place of ethanol precipitation. ACtually most plasmid extracts kits essentially employ the same principles of alkaline lysis. I still think alkaline lysis (done properly), is the best method to be used for plasmid preparation. I always used to get more DNA prep from alkaline lysis than from any other method.
> From: owner-methods at hgmp.mrc.ac.uk on behalf of Kyle Legate
> Sent: November 26, 2003 10:49 AM
> To: methods at hgmp.mrc.ac.uk
> Subject: Re: Plasmid extraction kits
> Cornelius Krasel wrote:
> > Please explain why Qiagen kits are faster than alkaline lysis. The
> > Qiagen kit that I know uses alkaline lysis to generate a crude DNA
> > preparation which is then further purified by column chromatography.
> > However, this further purification is often not necessary (it depends
> > on what you want to do with your DNA). So, if you stop right after
> > the alkaline lysis, you have Maniatis :-)
> Assuming you want some kind of purification step after the lysis, Qiagen
> kits are faster because they require four quick spins of 1 minute, compared
> to two much longer ethanol precipitation spins.
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