Smearing-when PCR product is used as template

Wolfgang Schechinger hubahopp at
Mon Oct 13 17:31:01 EST 2003

that is a very good thought. 
Are you aware of any source that summarizes all these PCR woes and teaches
how to overcome them? Maybe a book or website that does not only explain
the theory of PCR itself but emphasizes the theories of the errors that may
(and will) occur?



>Secondly, if you are doing multiple rounds of pcr you really need nested 
>primers to ensure you are reselecting only valid sequences. Nesting can 
>be difficult to achieve, but if you can only nest one end that is better 
>than nothing.
>Peter Ashby
>School of Life Sciences, University of Dundee, Scotland
>To assume that I speak for the University of Dundee is to be deluded.
>Reverse the Spam and remove to email me.


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