secrete pichia secrete

Stefanie Wedepohl stefanie.wedepohl at
Fri Oct 24 10:58:45 EST 2003

are there any tricks for forcing pichia pastoris to secrete my favourite
protein, if it doesn´t want to?

(given i cloned mfp in frame with secretion signal etc.. there is no formal
reason why it shouldn´t work , but it doesn´t)

thanx for all suggestions

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