Homemade DNA ladder?
Michael L. Sullivan
mlsulliv at facstaff.wisc.edu
Wed Sep 3 09:07:23 EST 2003
>Noticing that my lab is using an awful lot of DNA ladder, and that
>it's has gotten very expensive
>from the companies (especially now that certain companies have
>merged with or been absorbed
>Wondering whether everyone out there is just buying it, or making
>their own. Lambda/HindIII is
>OK but of course it would be better to have something with standard
>sizes, a bunch of sizes in the
>lower range, etc.
>Is there an easy way to make a nice one of your own?
Double digestion of lambda with EcoRI and HindIII gives a really nice
range of fragments for the larger range. Not sure about the lower
I have to admit, I routinely now by premade ladders. The one I use
most often is the 1 kb ladder from NEB (no affiliation) which ends up
costing about $0.25/lane (assuming a load of 0.5 ug). I do like the
convenience and having very regular sizes to the markers.
Michael L. Sullivan, Ph.D
Research Plant Molecular
U.S. Dairy Forage Research Center
1925 Linden Drive West
Madison WI, 53706
(608) 264-5397 Phone
(608) 264-5147 Fax
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