Homemade DNA ladder?
dk at no.email.thankstospam.net
Wed Sep 3 09:37:11 EST 2003
In article <email@example.com>, mlsulliv at facstaff.wisc.edu ("Michael L. Sullivan") wrote:
>>Noticing that my lab is using an awful lot of DNA ladder, and that
>>it's has gotten very expensive
>>from the companies (especially now that certain companies have
>>merged with or been absorbed
>>Wondering whether everyone out there is just buying it, or making
>>their own. Lambda/HindIII is
>>OK but of course it would be better to have something with standard
>>sizes, a bunch of sizes in the
>>lower range, etc.
>>Is there an easy way to make a nice one of your own?
>Double digestion of lambda with EcoRI and HindIII gives a really nice
>range of fragments for the larger range. Not sure about the lower
>I have to admit, I routinely now by premade ladders. The one I use
>most often is the 1 kb ladder from NEB (no affiliation) which ends up
>costing about $0.25/lane (assuming a load of 0.5 ug). I do like the
>convenience and having very regular sizes to the markers.
Then switch to MBI Ferments 1 kb ladder. It's the same as NEB but
has one more additional band, two reference band, costs less
($0.16/lane in your case) and and is available from on-campus
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