Homemade DNA ladder?

D.K. dk at no.email.thankstospam.net
Wed Sep 3 09:37:11 EST 2003


In article <p05200f01bb7ba34f1c5e@[192.168.80.1]>, mlsulliv at facstaff.wisc.edu ("Michael L. Sullivan") wrote:
>>Hi everybody,
>>
>>Noticing that my lab is using an awful lot of DNA ladder, and that 
>>it's has gotten very expensive
>>from the companies (especially now that certain companies have 
>>merged with or been absorbed
>>by others).
>>
>>Wondering whether everyone out there is just buying it, or making 
>>their own.  Lambda/HindIII is
>>OK but of course it would be better to have something with standard 
>>sizes, a bunch of sizes in the
>>lower range, etc.
>>
>>Is there an easy way to make a nice one of your own?
>>
>>Thanks!
>>
>
>Double digestion of lambda with EcoRI and HindIII gives a really nice 
>range of fragments for the larger range.  Not sure about the lower 
>range.
>
>I have to admit, I routinely now by premade ladders.  The one I use 
>most often is the 1 kb ladder from NEB (no affiliation) which ends up 
>costing about $0.25/lane (assuming a load of 0.5 ug).  I do like the 
>convenience and having very regular sizes to the markers.

Then switch to MBI Ferments 1 kb ladder. It's the same as NEB but
has one more additional band, two reference band, costs less 
($0.16/lane in your case) and and is available from on-campus 
freezers here.

DK
 



More information about the Methods mailing list