Silver staining Western membranes
tatjana.schwabe at ruhr-uni-bochum.de
Fri Sep 26 13:17:04 EST 2003
There are two papers I remember, both in Analytical Biochemisty.
Phelps (1984) describes a complete destaining procedure before the blot.
Perides (1986) mentions that transfer is not inhibited by CBB, but
describes a destaining step after transfer for better visualisation of
proteins on the blot.
I haven't tried any of the protocols myself though.
Sean Patterson wrote:
>> Never did a western transfer after staining with CBB-R. I have not
>> done it
>> because I believe the blot will be useless for proper immunodetection
>> if the
>> polypeptides are stained. However, Transferring ponceau stained gels has
> If I recall correctly, the gels were completely destained before
> transfer, but it's been so long since I looked at the original paper
> that I'm really not sure.
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