Dot blot on bacteria

Mélanie Tremblay mel_4_7 at yahoo.ca
Wed Apr 7 20:25:59 EST 2004


Hi,
I have to screen many clones of many expressed proteins in BL21 DE3 after
induction with IPTG.

Does someone already try to make a dot blot for screening of expressing
clones? I think that I we would be more easier and simple instead of making
many SDS PAGE. But I'm not sure about the method to lyse bacteria. Do you
think SSB will be sufficient before droping in nitrocellulose? (and probe
with a anti-his tag)

Thanks
Mélanie Tremblay





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