transformation hemi-methylated DNA

elmm1h at elmm1h at
Thu Apr 15 10:29:56 EST 2004

transformed DNa into DAM- strain in order to digest with BCLi Need to religate cleaved DNA, which will now be hemi methylated. Iam transforming back into a DAM+ strain but I am getting no colonies. Is the transformation of hemi-methylated DNA poor compared to methylated DNA?

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