transformation hemi-methylated DNA

elmm1h at udcf.gla.ac.uk elmm1h at udcf.gla.ac.uk
Thu Apr 15 10:29:56 EST 2004


transformed DNa into DAM- strain in order to digest with BCLi Need to religate cleaved DNA, which will now be hemi methylated. Iam transforming back into a DAM+ strain but I am getting no colonies. Is the transformation of hemi-methylated DNA poor compared to methylated DNA?

http://biowww.net/mynews/tree.php?group_name=bionet_molbio_methds-reagnts&begin=0



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