HeLa and Cos7 spontaneously chnaging phenotype???
chmc at chmc.org
Thu Apr 15 15:29:34 EST 2004
"D.K." <no.email at thanks.to.spam.net> wrote in message
news:c5mhv9$olo$1 at news.doit.wisc.edu...
> In article <Pine.SOL.4.58.0404151332510.18468 at yellow.csi.cam.ac.uk>,
"jg374 at cam.ac.uk" <jg374 at hermes.cam.ac.uk> wrote:
> >Somone in my lab has just had their HeLa and Cos7 cells spontaneously
> >into fibroblast-like cells within one passage. They havent used any new
> >media or anything. This also happened last summer in our lab to some
> >HeLas, where several people's HeLa's changed within a few days. The only
> >thing I can think of is some mycoplasma or virus type thing, but i'm
> >totally lost as to an explanation????
> The simplest explanation would be contamination by fibroblasts that
> outgrew these cultures. I know, not terribly believable either ...
> In many years, I've never seen HeLa or Cos changing phenotype.
> Mycoplasma would not do it but if it's a virus, you should find out
> and study it - very interesting!
Either that or someone mislabeled the vial. I'd probably buy a new stock
and go from there. You can drive yourself crazy trying to figure out
exactly what the evil gods of science are doing to you.
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