HeLa and Cos7 spontaneously chnaging phenotype???

chmc chmc at chmc.org
Thu Apr 15 15:29:34 EST 2004


"D.K." <no.email at thanks.to.spam.net> wrote in message
news:c5mhv9$olo$1 at news.doit.wisc.edu...
> In article <Pine.SOL.4.58.0404151332510.18468 at yellow.csi.cam.ac.uk>,
"jg374 at cam.ac.uk" <jg374 at hermes.cam.ac.uk> wrote:
> >Somone in my lab has just had their HeLa and Cos7 cells spontaneously
turn
> >into fibroblast-like cells within one passage. They havent used any new
> >media or anything. This also happened last summer in our lab to some
> >HeLas, where several people's HeLa's changed within a few days. The only
> >thing I can think of is some mycoplasma or virus type thing, but i'm
> >totally lost as to an explanation????
> >
>
> The simplest explanation would be contamination by fibroblasts that
> outgrew these cultures. I know, not terribly believable either ...
> In many years, I've never seen HeLa or Cos changing phenotype.
> Mycoplasma would not do it but if it's a virus, you should find out
> and study it - very interesting!
>
> DK
Either that or someone mislabeled the vial.  I'd probably buy a new stock
and go from there.  You can drive yourself crazy trying to figure out
exactly what the evil gods of science are doing to you.





More information about the Methods mailing list