Cloning by Overlap PCR

Neeresh Rohitlall NRohitlall at csir.co.za
Mon Apr 19 02:51:33 EST 2004


Hello,

 does anyone have a good protocol for mutagenesis using Overlap PCR?The
idea is to PCR the gene from the mutation site (forward and reverse from
the middle of the gene where mutation is to introduced) and then use the
the sequencing primers to pcr the whole gene which should include the
inserted mutaion. Does this make sense?am new to PCR strategies.
many thanks in advance
N Rohitlall
South Africa

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