Magnetic Bead Cleanup of BigDye Terminator Reactions

Mick Jones m.d.jones at imperial.ac.uk
Thu Apr 22 05:42:17 EST 2004


Hi

Has anybody had experience of using the CleanSEQ magnetic beads from
Agencourt to clean up their BigDye terminator sequence reactions before
loading onto a capillary sequencer (ABI 3700)?

How does it compare to Gel filtration columns?

Any comments gratefully appreciated.

Mick

Michael D Jones, BSc, PhD, ILTM
Head Genomics Core Laboratory
MRC Clinical Sciences Centre
Hammersmith Hospital Campus
London, UK 


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