imager for "quantitative" ECL detection--recommendation?
Michael L. Sullivan
mlsulliv at facstaff.wisc.edu
Wed Apr 28 14:21:23 EST 2004
I've been using Bio-Rad's Chemidoc system (no affiliation) both for
gel documentation and also for chemiluminescent detection for Western
blots using CDP-star chemistry. I only occasionally do
quantification, but that has worked reasonably well-- the limitations
had more to do with the limitations of quantifying western blots than
on the instrumentation.
>We're looking at buying a CCD gel imager for ECL detection and possibly for
>gel documentation. Does anybody have a recommendation for a unit that allows
>for quantification of ECL bands and saves nice quality tifs? I remember
>using a Kodak image station in the past but since the camera wasn't cooled,
>the background was high. Is a cooled CCD camera worth paying for?
Michael L. Sullivan, Ph.D
Research Plant Molecular
U.S. Dairy Forage Research Center
1925 Linden Drive West
Madison WI, 53706
(608) 264-5397 Phone
(608) 264-5147 Fax
More information about the Methods