PCR problems

rahul.ranjan at biocon.com rahul.ranjan at biocon.com
Wed Aug 4 02:35:14 EST 2004


At higher PCR cycles we observe degradation in all the samples (including the blank) in the form of smear (streaks). Although at lower cycles we observe a sharp band. Higher cycles are need to obtain an amplification of 1pg DNA (genomic DNA).

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