PCR problems

Kyle Legate legatek at hotmail.com
Wed Aug 4 12:17:34 EST 2004


rahul.ranjan at biocon.com wrote:
> At higher PCR cycles we observe degradation in all the samples
> (including the blank) in the form of smear (streaks). Although at
> lower cycles we observe a sharp band. Higher cycles are need to
> obtain an amplification of 1pg DNA (genomic DNA).
>
What could possibly be degrading in your blank. I think this is a larger
problem.





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