Cresol red for PCR loading buffer...

Kyle Legate legatek at hotmail.com
Thu Aug 5 12:47:10 EST 2004


Tim Fitzwater wrote:
> There are published reports of two different red dyes used for this
> purpose.
>
> 1) 0.01% Phenol red (R. J. Klebe, S. A. Rodriguez, M. L. VerBeek and
> T. A. Giambernardi, 1999. Simple method for "hot-start" RT-PCR.
> BioTechn. 27(6):1108-1110.)
> 2) Carl Wittwer's laboratory uses 0.1 mM Cresol Red for the same
> purpose (The Rapid Cyclist, Spring, 1994, page 9).
> Neither dye will act as a loading buffer, but serves as an indication
> that the Taq has been added and mixed properly.
>
Why would someone care enough to go to such lengths? Normal convection
during the cycles is more than enough to mix the enzyme.





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