sorbitol/betaine system revisited
wang at lebs.cnrs-gif.fr
Thu Aug 12 14:02:42 EST 2004
wang at lebs.cnrs-gif.fr (Chunguang Wang) wrote in message news:<1967cc70.0408120618.71529 at posting.google.com>...
> The first trial today showed that the growth in this medium is really
> slower than in normal LB (E. coli strain BL21(DE3)pLysS with pET
> plasmid). Why? and more importantly, how to do with it? I looked
> through some papers saying that sorbitol/betained is supplemented just
> before induction. But that also means that the LB, or whatever medium,
> will be diluted by sorbitol at least, since the concentration of
> sorbitol is not so low. Is that OK for the E. coli?
Aha, of course the growth of E.col is not so easy because the
enviroment is not so comfortable as normal LB. What a stupid question!
Just be patient until the OD600 is 0.6 or sth like this!?
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