sorbitol/betaine system revisited

Chunguang Wang wang at lebs.cnrs-gif.fr
Thu Aug 12 14:02:42 EST 2004


wang at lebs.cnrs-gif.fr (Chunguang Wang) wrote in message news:<1967cc70.0408120618.71529 at posting.google.com>...
> > 
> The first trial today showed that the growth in this medium is really
> slower than in normal LB (E. coli strain BL21(DE3)pLysS with pET
> plasmid). Why? and more importantly, how to do with it? I looked
> through some papers saying that sorbitol/betained is supplemented just
> before induction. But that also means that the LB, or whatever medium,
> will be diluted by sorbitol at least, since the concentration of
> sorbitol is not so low. Is that OK for the E. coli?
> 
Aha, of course the growth of E.col is not so easy because the
enviroment is not so comfortable as normal LB. What a stupid question!
Just be patient until the OD600 is 0.6 or sth like this!?



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