Introduction of restriction sites via extended primers?

Peter Frank peter_frankde at yahoo.de
Fri Aug 13 16:54:13 EST 2004


I wrote:

>... For the primer-template hybrids the overhang will be irrelevant
>and not affect the annealing temperature.

I have to correct myself or at least be more specific. It will not be
relevant for the very first primer-template hybrids with the template
being the molecules one introduced into the PCR mix.
But for newly synthesized amplicons serving as template it will affect
the annealing temperature.

Peter



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